Woven composites inevitably suffer from internal damage caused by low-velocity impact (LVI) during service, resulting in degradation of material properties. In this paper, the damage behavior of twill woven thermoplastic composites (TWTC) at LVI was investigated by a combination of test and simulation methods. A micro, meso and macro serial multi-scale model was constructed to predict the damage behavior of TWTC at LVI, and tests were conducted under 5 and 10J impact energy to verify the correctness of the multi-scale model. The results showed that the micro, meso and macro serial multi-scale model could accurately predict the impact damage behavior of the TWTC. Furthermore, damage occurred on both the front and back of the material under the large impact energy, and the damage was mainly caused by fiber fracture. Besides, the force response curves predicted by the numerical simulation of LVI were in good agreement with the test results, and the error of the damage area of the numerical simulation was within 10%.