Poor mechanical properties was the major factor restricting the development of room temperature vulcanized silicone rubber application， the structure of crosslinking agent on the mechanical properties had important influence. In order to improve the mechanical properties of room temperature vulcanized silicone rubber， a kind of modified crosslinking agent （E44-APS） was synthesized by epoxy resin E-44 and ammonia propyl three alkoxy silane as raw material through ring opening reaction. The structure of E44-APS was determined by the infrared spectrum， nuclear magnetic hydrogen spectrum.The tensile properties， curing properties，contact angle， surface morphology，thermal stability of the room temperature vulcanized silicone rubber was studied. The experimental data show that with E44-APS content increases， the tensile strength of room temperature vulcanized silicone rubber increase first and then decrease.When E44-APS content is 10%， the tensile strength and elongation at break can be up to 0.47 MPa and 306%. Curing time increases with the increase of E44-APS content，5% heat temperature loss decreases with increasing content of E44-APS.With the increase of E44-APS content， contact angle reduces first and then increases. The results show that the E44-APS can effectively improve the mechanical properties of room temperature vulcanized silicone rubber， will also bring some negative impacts， such as curing time prolong and thermal stability and hydrophobic descent.