The conductive silicone rubber were prepared with the conductive filler of Ag/Ni/glass microsphere.The morphology of conductive silicone rubber was characterized. The electrical property,electromagnetic shielding property and mechanical property of conductive silicone rubber were studied. The results illustrated that the shielding effectiveness of silicone rubber with Ag/Ni/glass microsphere increased in low frequency due to the presence of nickel,the electric conductivity and mechanical property of conductive silicone rubber were excellent and the electromagnetic shielding effectiveness was (70.6 to 98.8)dB in frequency of 10kHz to 6GHz when the volume fraction of filler was 56%.