Tool wear was studied using orthogonal experiment method while milling titanium alloy TB6 in different milling parameters. The flank face wear was measured by the photomicrograph after a certain period of milling during the milling process. The regression equation of TB6 tool wear was established by the experimental data. The experimental data indicated that the tool wear mainly occurred on the flank face and mostly showing a banded wear. The length of the band increases with the cutting speed increases. The brittle failure was occrured and the adhension of titanium was found on the tool face. The parameters effect on the tool life were cutting speed, feed speed, cutting width and cutting depth in turn. The regression equation has good regression variance and can predict the tool life well.